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主 Ninagaw日 et日1./Cryobiology73(2016)20-29
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Invertedmicroscope Fig.1.Schematicilustrationofthecryomicroscopicsystemusedinthisstudy目
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(A)t'=0ms (B)t'=3ms (C)t'=8ms
(D)t'=13ms (E)t'=20ms (F)t'=31ms
Fig.2.Progresofintracelularfrezingintheepidermaltisueofastrawberygeranium(Saxifragロ， stoloniferaCurtis)leafatacolingrateof-1oCfmin.Thetemperaturewas aproximately-80，( Theseimageswereprocesedusingfiltering，andthecontrastandbrightneswere叫 ustedusingimageanalysissoftware.Boldwhitearow:10日 tionwhere theintracelularicecrystalapeared;smalarows:directionofprogresintheformationofintracelularicecrystals;whitebar:25μm;t':timeelapsed(ms)sincethestartof intracelularfrezing.(A)t'=0ms，(B)t'=3ms，(C)t'=8ms，(0)t'=13ms，(E)t'=20ms，and(F)t'=31ms.
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