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Figure7MicrophotographsofA549celsa杭erexposureto thecombustiongasgeneratedfromABS(X40).
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indistinguishab1ecornpared to contro1 gas日Ihile A549 celswereexposedtotheefluentsproducedfrornABS，it apearedthatthecelsbegantoshrinkinsizeandcels wereiregu1ar1yshapedandhadobscurecelboundaries
(4) Thecelviabi1ityandlive/deadcelcountshowedthat A549celsprogresed企orn84%viab1eattirne0.5hto1es than20%viab1eby4hours.Thefolowingtoxicityranking asobserved企omthemosttoxictothe1easttoxic:ABS> Ny1ons>Urethane>Me1amine.
Acknowledgement Weapreciatethatthispaperispartialysuportedbyfundsof
Grant-in-AidforScientificResearchNo.20510167inJapan References
[l]Japan Asociation for Fire Science and Enginering， Handbook ofFire (Japanese tit1e:Kasai-Binran)，Kyoritsu Shuppan，Tokyo，197，p.136-236.
[2]H.Naito，Poisoningofindustria1products，gases，pesticides，
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四
5 25
104-1IL Jし. . .JL JL JILJL J1ー. . .JL JILJL JL JL. . .JI←
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exposuretocombustiongasfromABS.
Oh '0.5h' 2h '4h 1Oh 'O.5h' 2h '4h 1Oh 'O.5h' 2h '4h |lll
cOz Air Firegas
Figure 8Cel l viability and cel l live/dead cel l count after
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Figure9Cel viability and cel live/dead cel count after exposuretocombustiongasfromABS，nylons，Urethane andMelamine.
4 Conclusions
Cytotoxicityandrnorpho1ogica1asesrnentofcornbustionby- products 企orn four p1astics:acry1onitrile-butadiene-styrenes (ABS)，ny1ons，UrethaneandMe1amine，inhurnan1ungderived cels(A549)，havebeninvestigated
(1) Thefiregascolectionandhurnancelexposuresystern werenew1ydeve10pedtoofersana1temativernethodto 1aboratoryanirna1s.Thehumancelexposuresysterncan enab1eA549，ahurnan1unga1veo1artypeIcel1ine，to ap1ythrediferentincubationgases，5%CO2 (contro1 celcu1tureatrnosphericcondition)，air(79%nitrogenand 21% oxygen)andthecornbustiongas.
(2) ThefolowingisHCNgenerationorderasrneasuredforn the rnostproductive to 1east productive: ABS>Urethane >Ny1onrneasuredbygaschrornatograph.Thegasdetector tube systerns with short-tern quick-rneasuring detector tubesshowedthatCOandHCHOofUrethaneis1arger than Ny1on， whi1e H C N production by Ny10n is 1arger 白 anUrethane.
(3) Folowingexposureto95%airwith5%CO2，afterthe timeperiodsof24，48and72h，nornorpho1ogica1change wasobservedintheA549.Thecelsexpos町 etoa廿 showed that cel boundaries are obscure and or
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[13]P. C. Burcham，A. Raso，C.A. Thornpson，Chernico-
424
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・ Bio1ogica1lnteractions，183(3)(2010)416