Page 37 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

I- Colorimetry

 It is one of the instrumental methods of analysis that depends
on measuring the interaction between electromagnetic
radiation (EMR) and molecules or atoms.

 It is used for structural and quantitative analysis of
Spectrophotometry
substances.

 (spectro- = spectrum), (photo- = light or photon),
(-metry = measurement)

{measuring light spectrum}

 It is the method for quantitative analysis which depends on
Colorimetry measuring the absorption of visible radiation by the system

to be determined.

U.V. region 180-380 nm.

Visible region 380-780 nm.

How we see colored things
!!!
Absorbed and reflected color

9 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5