Page 43 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

Calculations:

Conc. of stock solution = 0.1 mg % .
You take 1 mL from this solution then dilute to 100 mL.
C V = C' V'
0.1 x 1 = C' x 100
-3
C' = 1 x 10 gm%.
With the same manner, convert 3, 6, 9, 10 mL from the stock to its equivalent
concentration.

Volume
of stock Conc. (gm%) Absorbance
(mL)
2 .. ................. ......... .. ..........................
4 .. .......................... .. ..........................
6 .. .......................... .. ..........................
8 .. .......................... .. ..........................

10 . ...... ..................... .. ..........................

Slope = 1% = A2 – A1
 cm 1 C2 – C1

 The concentration obtained from the calibration curve is the concentration of
diluted sample and dilution factor should be considered

100
original conc. of the sample = Conc. obtained from the calibration curve x
10

dilution
factor = 10

15 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5