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Antibiotic Sensitivity Discs and Polydiscs-ESBL, Carbohydrate Fermentation Discs,Polydiscs
Code No Agent Symbol Level (μg) Code No Agent Symbol Level (μg) ASDP
EP5A Ampicillin + Sulbactam SAM 20 EP7 Amoxicillin/ Sulbactum AMS 30/15
Cefazolin CZ 30
For Gram For Gram Ampicillin/ Cloxacillin AX 10
Nega ve Cefuroxime CXM 30 Posi ve Cocci
Teicoplanin TP 30
Bacilli from
Chloramphenicol C 30 Resistant to
Urine Common Tigecycline TGC 15
Levofloxacin LVX 5 An bio cs
Moxifloxacin MO 5
1 Pack of
Nalidixic acid NA 30
10 Rings for 1 Pack of Cefpodaxime CEP 10
Rs 300/‐ Nitrofurantoin F 300 10 Rings for Lomefloxacin LOM 15
Norfloxacin NOR 10 Rs 300/‐
1 Pack of Meropenem MEM 10
20 Rings for Ofloxacin OFX 5 1 Pack of Enrofloxacin ENR 5
Rs 500/‐ 20 Rings for
Piperacillin PIP 100 Rs 500/‐ Oleandomycin OL 15
Piperacillin Novobiocin NV 30
TZP 110
+Tazobactum Spiramycin SP 100
Tobramycin TM 10
Code No Agent Symbol Level(μg)
Code No Agent Symbol Level (μg) EP8 Cefepime/
CPT 80/10
EP6A Cefazolin CZ 30 For Tazobactum
Gram Cefixime/ Clavulanic
CMC 5/10
For Cefuroxime CXM 30 Nega ve acid
Gram Posi ve Resistant to Ce riaxone/
Ciprofloxacin CIP 5 CIS 30/15
Cocci from Common sulbactum
urine Erythromycin E 15 An bio cs Ce riaxone/
Tazobactum CIT 80/10
Levofloxacin LVX 5
1 Pack of 1 Pack of Imipenem/ Cilas n IC 10/10
10 Rings for Nalidixic acid NA 30 10 Rings for Meropenem
MEM 10
Rs 300/‐ Rs 300/‐
Nitrofurantoin F 300 Cefoperazone +
SCF 50/50
1 Pack of Oxacillin OX 1 1 Pack of sulbactum Antibiotic Sensitivity Discs and Polydiscs-Polydiscs Pack of 20 Rings
20 Rings for Penicillin P 10 20 Rings for Ce azidime/ CAC 30\10
Rs 500/‐ Rs 500/‐ Claulanic Acid
Roxithromycin RXT 30 Nadifloxacin NAD 5
Tetracycline TE 30 Lomefloxacin LOM 15
Vancomycin VA 30 Prulifloxacin PR 10
Aztreonam ATN 50
Because of the ease of performance and the flexibility of the test, Kirby‐Bauer method of the disc diffusion is most widely used technique throughout the world for an bio c
sensi vity tes ng.
Medium‐Mueller‐Hinton agar is the recommended medium because it produces rapid growth of most of the pathogens, contains no inhibitors, can be used with sulpha
compounds and it gives a sharp end point, it should be supplemented with 5% defibrinated blood for fas dious organisms.
Procedure‐Pick up 3‐5 isolated colonies and suspend in 5ml of any suitable broth such as tryptone soya broth. Incubate the inoculated broth for 3‐5 hrs to get just visible turbidity.
It should be comparable to opacity of 0.5 McFarland nephelometer standards. If less turbid, incubate further, if more turbid, dilute with sterile normal saline. Dip a sterile co on
swab in the broth; remove extra broth by rota ng the swab against the wall of the tube. Swab Mueller‐Hinton agar plate thoroughly by turning the plate. Leave the swabbed plate
at the room temperature for 3‐5 minutes to dry the innoculums. Pick up individual disc or polydisc ring with flamed forceps and put on the swabbed medium with gentle press.
Discs should be at least 18‐24 mm apart from each other. Polydisc should be put in the centre of the plate. Incubate the plate at 37 c for a minimum of 18 hrs. Measure the zone
of inhibi on and interpret the result using the chart.
0.5 McFarland turbidity standard: Add 0.5 ml of 0.048 M: Bacl2 to 99.5 ml of 1% H2SO4. This standard should be replaced monthly.
Precau on‐Store the discs at 4 c in a refrigerator. Remove the discs at least 1 hour prior to use. Discs should be held at room temperature for as short a me as possible. Overlook
the swarming of Proteus spp. Depth of the medium should be 4 mm.
Note‐Sensi ve indicates that an bio c therapy at the recommended level will be successful. Resistant indicates that micro‐organisms are not suscep ble to achievable drug level.
Moderately Sensi ve indicates micro‐organisms are suscep ble to therapy if the upper limit of an bio c dosage is employed.
Antibiotic Sensitivity Discs and Polydiscs-Polydiscs Pack of 20 Rings
Interpreta ve zone diameter is different for each agent because of the varia on in disc potency. Ph, depth of the medium, inoculums, and age of the culture influence the result.
In case where long term therapy is required like SBE, it is necessary to know bactericidal level of the an bio c. Cephalothin can be used to predict ac vity of Cephradine,
Cephalexin, Cefaclor & Cefadroxil. Tetracycline is the representa ve of all tetracyclines. Ampicillin is the close representa ve for Ampicillin and Amoxicillin. Ampicillin results may
be used to predict suscep bility to Amoxicillin‐Clavulanic Acid, Ampicillin‐Sulbactum, Piperacillin and Piperacillin‐Tazobactum among non β‐lactamase producing enterococci.
Clindamycin disc is used for tes ng suscep bility to both Clindamycin and Lincomycin. Of the an ‐staphylococcal, β‐lactamase resistant penicillins either Oxacillin or Methicillin
could be tested and results could be applied to both and to Cloxacillin. Oxacillin is preferred due to more stability during storage. Suscep bility to Nalidixic Acid, Nitrofurantoin
and Norfloxacin apply to organisms isolated from UTI. For Salmonella spp.& Shigella spp. aminoglycosides may appear ac ve in vitro, but are not effec ve clinically and should
not be reported as sensi ve. For spp. of salmonella & shigella first and second genera on cephalosporins may appear ac ve in vitro, but are not effec ve clinically and should not
be reported as sensi ve. Sulfisoxazole (sulphafurazole) discs can be used to represent any of the currently available sulphonamide prepara ons. Erythromycin is class
representa ve of Azithromycin, Clarithromycin and Diarthromycin.
O
On receipt store below -20 C Pg No
Each Vial contains 100 Antibiotic Sensitivity Discs
LABORATORIES Each Cartridge contains 50 Antibiotic Sensitivity Discs 87
Email Id:eoslab@yahoo.com Packing of 50 Discs per Vial also available
Contact No: +91 9892652032
+91 8369991445 Antibiotic Sensitivity Discs and Polydiscs-Polydiscs (20 Rings)