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Antibiotic Sensitivity Discs and Polydiscs-ESBL, Carbohydrate Fermentation Discs,Polydiscs








       Code No     Agent                Symbol   Level (μg)         Code No   Agent             Symbol   Level (μg)       ASDP

       EP5A        Ampicillin + Sulbactam   SAM    20          EP7          Amoxicillin/ Sulbactum   AMS   30/15

                   Cefazolin              CZ       30
       For Gram                                                For Gram     Ampicillin/ Cloxacillin   AX    10

       Nega ve     Cefuroxime            CXM       30          Posi ve Cocci
                                                                            Teicoplanin           TP        30
       Bacilli from
                   Chloramphenicol        C        30          Resistant to
       Urine                                                   Common       Tigecycline          TGC        15
                   Levofloxacin           LVX        5          An bio cs
                                                                            Moxifloxacin          MO         5
       1 Pack of
                   Nalidixic acid        NA        30
       10 Rings for                                            1 Pack of    Cefpodaxime          CEP        10
       Rs 300/‐    Nitrofurantoin         F        300         10 Rings for   Lomefloxacin        LOM        15
                   Norfloxacin            NOR       10          Rs 300/‐
       1 Pack of                                                            Meropenem            MEM        10
       20 Rings for    Ofloxacin          OFX        5          1 Pack of    Enrofloxacin          ENR        5
       Rs 500/‐                                                  20 Rings for
                   Piperacillin          PIP       100            Rs 500/‐   Oleandomycin         OL        15

                   Piperacillin                                             Novobiocin            NV        30
                                         TZP       110
                   +Tazobactum                                              Spiramycin            SP       100
                   Tobramycin            TM        10
                                                                 Code No   Agent              Symbol    Level(μg)

       Code No     Agent                Symbol   Level (μg)    EP8         Cefepime/
                                                                                                CPT      80/10
       EP6A        Cefazolin             CZ        30          For         Tazobactum
                                                               Gram        Cefixime/ Clavulanic
                                                                                               CMC       5/10
       For         Cefuroxime            CXM       30          Nega ve     acid

       Gram Posi ve                                            Resistant to   Ce riaxone/
                   Ciprofloxacin          CIP        5                                           CIS      30/15
       Cocci from                                              Common      sulbactum

       urine       Erythromycin           E        15          An bio cs   Ce riaxone/
                                                                           Tazobactum           CIT      80/10
                   Levofloxacin           LVX        5
       1 Pack of                                               1 Pack of    Imipenem/ Cilas n   IC       10/10

       10 Rings for   Nalidixic acid     NA        30          10 Rings for   Meropenem
                                                                                               MEM        10
       Rs 300/‐                                                Rs 300/‐
                   Nitrofurantoin         F        300                     Cefoperazone +
                                                                                                SCF      50/50
       1 Pack of    Oxacillin            OX         1          1 Pack of    sulbactum                                  Antibiotic Sensitivity Discs and Polydiscs-Polydiscs Pack of 20 Rings
       20 Rings for    Penicillin         P        10          20 Rings for    Ce azidime/      CAC      30\10
       Rs 500/‐                                                Rs 500/‐    Claulanic Acid
                   Roxithromycin         RXT       30                      Nadifloxacin         NAD         5
                   Tetracycline           TE       30                      Lomefloxacin         LOM        15
                   Vancomycin            VA        30                      Prulifloxacin         PR        10
                                                                           Aztreonam            ATN       50
   Because of the ease of performance and the flexibility of the test, Kirby‐Bauer  method of the disc diffusion is most widely used technique throughout the world for an bio c
   sensi vity tes ng.
   Medium‐Mueller‐Hinton agar is the recommended medium because it produces rapid growth of most of the pathogens, contains no inhibitors, can be used with sulpha
   compounds and it gives a sharp end point, it should be supplemented with 5% defibrinated blood for fas dious organisms.
   Procedure‐Pick up 3‐5 isolated colonies and suspend in 5ml of any suitable broth such as tryptone soya broth. Incubate the inoculated broth for 3‐5 hrs to get just visible turbidity.
   It should be comparable to opacity of 0.5 McFarland nephelometer standards. If less turbid, incubate further, if more turbid, dilute with sterile normal saline. Dip a sterile co on
   swab in the broth; remove extra broth by rota ng the swab against the wall of the tube. Swab Mueller‐Hinton agar plate thoroughly by turning the plate. Leave the swabbed plate
   at the room temperature for 3‐5 minutes to dry the innoculums. Pick up individual disc or polydisc ring with flamed forceps and put on the swabbed medium with gentle press.
   Discs should be at least 18‐24 mm apart from each other. Polydisc should be put in the centre of the plate. Incubate the plate at 37 c for a minimum of 18 hrs. Measure the zone
   of inhibi on and interpret the result using the chart.
   0.5 McFarland turbidity standard: Add 0.5 ml of 0.048 M: Bacl2 to 99.5 ml of 1% H2SO4. This standard should be replaced monthly.
   Precau on‐Store the discs at 4 c in a refrigerator. Remove the discs at least 1 hour prior to use. Discs should be held at room temperature for as short a  me as possible. Overlook
   the swarming of Proteus spp. Depth of the medium should be 4 mm.
   Note‐Sensi ve indicates that an bio c therapy at the recommended level will be successful. Resistant indicates that micro‐organisms are not suscep ble to achievable drug level.
   Moderately Sensi ve indicates micro‐organisms are suscep ble to therapy if the upper limit of an bio c dosage is employed.
                                                                                                                       Antibiotic Sensitivity Discs and Polydiscs-Polydiscs Pack of 20 Rings
   Interpreta ve zone diameter is different for each agent because of the varia on in disc potency. Ph, depth of the medium, inoculums, and age of the culture influence the result.
   In case where long term therapy is required like SBE, it is necessary to know bactericidal level of the an bio c. Cephalothin can be used to predict ac vity of Cephradine,
   Cephalexin, Cefaclor & Cefadroxil. Tetracycline is the representa ve of all tetracyclines. Ampicillin is the close representa ve for Ampicillin and Amoxicillin. Ampicillin results may
   be used to predict suscep bility to Amoxicillin‐Clavulanic Acid, Ampicillin‐Sulbactum, Piperacillin and Piperacillin‐Tazobactum among non β‐lactamase producing enterococci.
   Clindamycin disc is used for tes ng suscep bility to both Clindamycin and Lincomycin. Of the an ‐staphylococcal, β‐lactamase resistant penicillins either Oxacillin or Methicillin
   could be tested and results could be applied to both and to Cloxacillin. Oxacillin is preferred due to more stability during storage. Suscep bility to Nalidixic Acid, Nitrofurantoin
   and Norfloxacin apply to organisms isolated from UTI. For Salmonella spp.& Shigella spp. aminoglycosides may appear ac ve in vitro, but are not effec ve clinically and should
   not be reported as sensi ve. For spp. of salmonella & shigella first and second genera on cephalosporins may appear ac ve in vitro, but are not effec ve clinically and should not
   be reported as sensi ve. Sulfisoxazole (sulphafurazole) discs can be used to represent any of the currently available sulphonamide prepara ons. Erythromycin is class
   representa ve of Azithromycin, Clarithromycin and Diarthromycin.

                                                O
                                 On receipt store below -20 C                                                   Pg No
                                 Each Vial contains 100 Antibiotic Sensitivity Discs

    LABORATORIES                 Each Cartridge contains 50 Antibiotic Sensitivity Discs                       87

    Email Id:eoslab@yahoo.com    Packing of 50 Discs per Vial also available
    Contact No: +91 9892652032

                       +91 8369991445   Antibiotic Sensitivity Discs and Polydiscs-Polydiscs (20 Rings)
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