Page 317 - SUBSEC October 2017_Neat

UNIT 2
MODULE 2: ANALYTICAL METHODS AND SEPARATION TECHNIQUES (cont’d)

SPECIFIC OBJECTIVES EXPLANATORY NOTES SUGGESTED PRACTICAL
ACTIVITIES

Spectroscopic Methods of Analysis cont’d

Students should be able to:

4.2. state the approximate Relative energies and
wavelength ranges of the dangers associated with
X-ray, UV/VIS, IR and exposure to high energy
radiofrequency regions of wavelengths.
the electromagnetic
spectrum; and, Insert actual ranges for x-
ray, UV, VIS, IR and RF.
4.3. recall that the energy levels Refer to Unit 1, Module 1.
in atoms and molecules are
quantised.

5. Ultra Violet-Visible (UV/VIS) Spectroscopy

Students should be able to:

5.1. explain the origin of Simple treatment based on
absorption in UV/VIS Unit 1, Module 1.
spectroscopy; Consideration of sigma (σ),
pi (π), anti-bonding (σ*,
π*) and non-bonding (n)
orbitals.
5.2. explain why some species
will absorb light in the
UV/VIS region whereas
others will not;

5.3. describe the basic steps Brief mention should be
involved in analysing made of the use of
samples by UV/VIS complexing reagents to
spectroscopy; form coloured
compounds. Sensitivity
and detection limits.

5.4. use Beer-Lambert’s Law to Use of standards and
calculate the concentration calibration curves.
of a given species in
solution; and,

5.5. list examples of the use of Iron tablets; glucose and
UV/VIS spectroscopy in the urea in blood; cyanide in
quantitation of substances. water.

CXC A11/U2/17 48