Page 22 - tech fest 2025 ב״ש חוברת תקצירים

22
Micronization Process Improvement: Obtaining Desired Particle Size Distribution (PSD) by Dry Jet Milling
ChE-D-06
Sergey Vorobiov; srgvorobiov@gmail.com
Advisors: Prof. Adi Wolfson1, Mr. Nir Sason2 1SCE - Shamoon College of Engineering, Be’er-Sheva 2Wavelength Pharmaceuticals, Ne'ot Hovav
This project focuses on optimizing the micronization of fluticasone propionate, a steroid used to treat asthma. A dry jet mill micronizer using high-pressure nitrogen gas jets was employed to achieve micron-range particles by inducing particle collisions. The process parameters were optimized for better efficiency and precise particle size control. Particle size distribution (PSD) was confirmed using X-ray powder diffraction (XRPD). Differential scanning calorimetry (DSC) was used to evaluate storage conditions and the potential transition to an amorphous state. Scanning electron microscopy (SEM) was used to visually compare crystalline and amorphous morphologies. This study emphasizes the importance of controlling micronization and stability parameters to ensure high-quality pharmaceutical powder suitable for inhalation.
Keywords: DSC, fluticasone propionate, inhalation therapy, micronization, milling process, PSD, SEM, XRPD
Testing Antimicrobial Properties of Endophytes from the Khat Plant
ChE-E-01
Shahd Khateb; khatebshahd8@gmail.com
Advisor: Dr. Yoram Shotland
SCE - Shamoon College of Engineering, Be’er-Sheva
Catha edulis, commonly named ‘khat’, is a shrub known for its psychopharmacological properties and notable resistance to disease. This resilience may be linked to ‘endophytes’ (microorganisms living within plant tissues) that exhibit antimicrobial activity. In this study, several endophytes were isolated from khat, and three strains (24, 25 and 30) were selected for further investigation. Their ability to inhibit environmental bacteria, as well as plant and human pathogenic bacteria, was tested. Disc diffusion assays show clear inhibition zones against all tested bacteria. Significant inhibition was also observed in the cell-free supernatant, suggesting active compound secretion; however, no inhibition was detected after filtration, indicating that the active compound may have been absorbed by the filter. Apparently, Khat’s endophytes contribute to its antimicrobial activity.
Keywords: antimicrobial activity, Catha edulis, disc diffusion assay, endophytes, pathogenic bacteria, plant-microbe interaction