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Materials and Nanotechnology | Progress Report 319

Figure 51. Fluorescence spectroscopy of bovine serum albumin biding to gold nanoparticles

(CD163 mimic) developed by Dr. Rosemeire this context, the seek for biologically-friend-
Silva for internalization studies in prostate ly materials are of highlighted role. Proteins
cancer cells and pancreatic cancer cells (Figure are biocompatible by nature and hold strong
50). We have also explored the chromophores biological affinity with a considerable lack
of Albumin as a possible internal dosimeter for of toxicity when administered to humans.
radiation therapy as follow bellow (Figure 51). Within this context, several studies have been
performed using proteins as nanocarriers. In
Development of protein based this work, we developed protein based nano-
nanocarriers for the delivery of carriers, using bovine serum albumin or papain
radiopharmaceuticals as model protein and enzyme respectively,
by the use of radiation. Unlike conventional
Nanoparticles and nanoparticulate systems pathways, radiation allows the sterilization
are considered a breakthrough in several fields and simultaneous crosslinking of the protein
as novel applications and features of common- in a single step, without the need for toxic
ly used materials were brought to light as a monomers or crosslinkers that commonly lead
result of the nanostructuration. Biopharma- to undesirable reactions when administered
ceutical and biomedical advantages, including to biological systems. The nanocarriers are
site specific delivery are good examples of being radiolabeled with 99mTc by the direct
advantages that are offered by nanotechnology and indirect method, in order to achieve an
over conventional systems. At the same pace, alternative system for the delivery of this and
concerns regarding toxicity emerge as most other radioisotopes for diagnose and treatment
molecules or nanoparticles have no biologi- of a variety of disorders. High radiolabeling
cal affinity, thus adverse reactions may take yields with good radiochemical stability have
place including non-specific toxicity. Within been identified throughout the experiments