Page 17 - KVIS Book

ENS09A

THE SYNTHESIS AND DEVELOPMENT OF MAGNETIC
NANOPARTICLES TEST KIT FOR HEAVY METAL DETECTION

Pimfa Kamkalong, Prairsunan Chanpanich
Kamnoetvidya Science Academy, Thailand
1
2
Supervisor: Suranan Anantachaisilp , Kanokorn Wechakorn
1 Kamnoetvidya Science Academy,
2 Rajamangala University of Technology Thanyaburi
Email: prairsunan_c@kvis.ac.th

One major form of water pollution is heavy metal contamination, which can lead to lethal
diseases. This research focuses on the synthesis and characterization of Rhodamine B
derivative sensors modified by the attachment of magnetic nanoparticles (RB-MNPs) for
heavy metal test kit. The synthesis section was divided into two parts: the Rhodamine B
derivative sensors (RB sensors) synthesis and the magnetic nanoparticles (MNPs) synthesis.
The RB sensors and MNPs were then attached together by a click reaction resulting
RBMNPs. The RB-MNPs were then used to detect various metal ions and characterized for
their physical properties and optical responses. RB-MNPs have spherical shapes and
diameters of 155 nm. They can detect Cu²⁺, Ni²⁺, Hg²⁺, and Co²⁺, resulting the solutions with
four specific shades of pink, and detect Fe²⁺, Fe³⁺, and Pb²⁺, resulting the solutions with
three specific shades of orange. The increment of fluorescent intensity wasdeveloped with
data gathered after the detection of these metals. Calibration curves showing the
relationships between several optical responses and Cu²⁺ concentrations were then
plotted. The curves were used to identify a 10 µM, or 0.6 ppm, limit of detection (LOD) for
Cu²⁺. The RB-MNP test kit developed for this study was then used to test for heavy metals
in water collected from canals in an industrial area in Samut Prakan, Thailand. The results
reveal that the kit can detect metals in water. Furthermore, a smartphone application was
developed using the RGB imaging technique in order to provide accurate results to users.

Keywords: Rhodamine B derivative sensors, Magnetic Nanoparticles, click reaction