Page 24 - KVIS Book

LIS05A

Antibacterial compound from durian peel extract

Jita Supradit, Penpitcha Sirisoongnern
Kamnoetvidya Science Academy, Thailand
Supervisor: Janjira Maneesan
Email: jita_s@kvis.ac.th

The purpose of “Antibacterial compound from durian peels project” is to study the
effectiveness of inhibiting bacterial growth of durian peel extract and to specify
antibacterial compounds in durian peel extracts. According to previous research,
durian peel has antibacterial property. In addition, durian peel is also a waste of
agricultural residue which has to be removed by incineration. If it can be used usefully,
pollution will be reduced. In this project, four pathogenic bacteria, Staphylococcus
aureus, Enterococcus faecalis, Aeromonas hydrophila, and Escherichia coli, are used to
test with crude extracts from Puangmanee, Montong, Chanee and Kanyao durian peel.
The experiments were divided into 3 methods, beginning with extraction of
antibacterial extract from durian peel which used distilled water, ethanol, and acetone
as solvents. Then, verified the efficiency of bacterial inhibition by agar diffusion assay.
Lastly, purify and specify antibacterial compound in crude extracts. The results show
that crude extracts from Monthong, Puangmanee, Chanee, and Kanyao have
antibacterial property which affect to different bacteria species. The crude extract that
seems the highest effectiveness in bacterial growth inhibition is the crude extract from
Monthong which used ethanol as solvent. It affects 3 bacteria species: A.hydrophila,
E.facalis, and S.aureus. For E.coli, there is only the extract from Chanee durian peel
which extracted by acetone can inhibit its growth. Resulting from TLC analysis,
compounds in Chanee durian peel extracts, which used ethanol 99% as solvent, are
possibly in alkaloid, flavonoid, or glycoside group.

Keywords: Durian, Antibacterial compound