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Bone Aspiration and Biopsy   1067


                                                                                  a small-gauge needle attached to a pressure
                                                                                  transducer or catheter placement in a distal
  VetBooks.ir                                                                     to pressure tubing and a pressure transducer. A
                                                                                  artery (typically dorsal pedal) and attachment
                                                                                  BP tracing is printed, and systolic and diastolic
                                                                                  pressure can be determined from the pressure
                                                                                  tracing.
                                                                                  •  Highly accurate information because direct
                                                                                    measurement is used
                                                                                  •  Local  anesthesia  is  used  to  minimize
                                                                                    discomfort.
                                                                                  •  Arterial puncture or arterial catheter place-
                                                                                    ment  requires  more  technical  skill  than
                                                                                    noninvasive methods (p. 1058).
                                                                                  •  Bleeding may occur at site of arterial punc-
                                                                                    ture; apply pressure to puncture site for at   Procedures and   Techniques
                                                                                    least 5 minutes after the needle is withdrawn.
                                                                                  •  Invasive methods are rarely used in conscious
                                                                                    clinical patients, but indwelling arterial
           BLOOD PRESSURE MEASUREMENT  Automated oscillometric method used with a forelimb cuff to obtain
           systolic, diastolic, and mean blood pressure in a dog. Dog is restrained in a comfortable position, with intended   catheters are frequently used for continuous
           cuff-site limb gently extended. Cuff is positioned at mid-radius level, and limb is positioned such that cuff is   BP assessment in anesthetized or critically
           at level of right atrium during readings.                                ill patients.
                                                                                  Pearls
                                                                                  Cuff location, cuff size, and animal position
             representative number for systolic, diastolic,   Postprocedure       can affect measured BP values. Each of these
             and mean pressures, respectively.  •  Record the average values in animal’s record,   should be noted for each patient and remain
           •  If heart rate associated with a reading is clearly   with notation of method, cuff size, and cuff   the same for future BP assessments.
             incorrect, BP values may be spurious. High   site used.
             heart rate during recording may indicate high   •  Evaluate BP values in light of clinical findings   SUGGESTED READING
             animal stress levels and possible elevated BP   and level of anxiety or excitement during BP   Brown S, et al: Guidelines for the identification, evalu-
             due to stress of procedure. If heart rate is   measurement.           ation, and management of systemic hypertension in
             decreasing during serial measurements, take                           dogs and cats. J Vet Intern Med 21:542-558, 2007.
             several more readings and average the final 5   Alternatives and Their    AUTHOR: Rebecca L. Stepien, DVM, MS, DACVIM
             values. If an arrhythmia is present, the heart   Relative Merits     EDITORS: Leah A. Cohn, DVM, PhD, DACVIM; Mark S.
             rate may not be recorded and the results of   Invasive BP measurement involves acute arte-  Thompson, DVM, DABVP
             that iteration should be discarded.  rial puncture (typically femoral artery) with







            Bone Aspiration and Biopsy                                                               Bonus Material
                                                                                                          Online


                                               Equipment, Anesthesia
           Difficulty level: ♦                                                    •  For biopsy: sterile gloves, #11 scalpel blade,
                                               •  Heavy  sedation,  neuroleptanalgesia,  or   Jamshidi needle or bone trocar, tissue jar,
           Synonym                              general  anesthesia with  local  analgesic for   and 10% formalin
           Bone fine-needle aspiration (FNA)    biopsy
                                                ○   Typical sedation protocol: dexmedetomi-  Anticipated Time
           Overview and Goal                      dine 5 mcg/kg IM or IV with morphine   15-20 minutes
           Minimally invasive sampling of bony mass or   0.5 mg/kg IM or IV
           lesion identified radiographically   ○   Typical general anesthesia protocol:   Preparation: Important
                                                  premedicate with above sedation drugs,   Checkpoints
           Indications                            induce with propofol 6 mg/kg IV to effect,   •  Radiographs:  confirm  lesion  and  best
           Used  to  obtain  diagnosis  of  bony  mass  or   maintenance with propofol as needed or   sampling location
           radiographic aggressive bone lesion (pp. 165,   isoflurane gas         •  Thoracic radiographs: to check for metastatic
           725, and 726). Simple aspiration often used   ○   Local analgesia: 9 : 1 mixture of 2% lido-  disease from primary bone tumors
           first, moving on to a biopsy if diagnosis is not   caine HCl to sodium bicarbonate 1-2 mL   •  Discussion with owner of potential complica-
           forthcoming.                           infiltrated subcutaneous to periosteum  tions, including iatrogenic pathologic fracture
                                               •  Clippers
           Contraindications                   •  Antiseptic scrub solution, isopropyl alcohol,   Possible Complications and
           •  Contraindication to sedation or anesthesia   gauze squares for skin preparation  Common Errors to Avoid
             (biopsy)                          •  For aspiration: sterile or nonsterile gloves,   •  Iatrogenic pathologic fracture: altering angle
           •  Severe bleeding disorder          hypodermic needles (18 or 20 gauge), 10-mL   or location of multiple samples may reduce
           •  Skin infection over bone sampling site  syringe, cytology slides (#10-#20)  risk.

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