Page 567 - The Veterinary Laboratory and Field Manual 3rd Edition
P. 567

504  Samuel Sharpe

            SaMPLE coLLEctIon                        •  Presence of fluid or material including sta-
            •  Take a transverse section including mucosa   ble foam, purulent or blood tinged exudate,
              after bread loafing and place in formalin.  or food material within the tracheal lumen
                                                       should be noted.
                                                     •  The mucosa should be shiny, intact, and
            Oesophagus
                                                       translucent. Mucosal defects, ulcers, vesicles,
            •  Using scissors and forceps open the oesopha-  nodules, areas of congestion or haemorrhage
              gus along its length.                    should be noted.


            tHInGS to notE                           SaMPLInG
            •  The mucosa should be homogenously tan,   •  Take a 2 cm long section of opened trachea
              slightly undulating, soft, with an intact, shiny   into formalin.
              surface. Mucosal defects, ulcers, vesicles,   •  Take a cross section of lymph node includ-
              nodules, areas of congestion or haemorrhage   ing the capsular surface, cortex, medulla and
              or masses should be noted and sampled.   hilus.
            •  The submucosa should be abundant and
              loose. Masses, strictures or foreign bodies
              should be noted.                       Thyroids and parathyroids
            •  The muscularis is comprised of soft to firm,   •  Thyroid and parathyroid anatomy varies quite
              pale pink smooth muscle. Masses or foreign   significantly between domestic species; an
              bodies should be noted.                  anatomy text should be consulted if the pro-
                                                       sector is unfamiliar.
            SaMPLInG                                 •  Thyroids and parathyroids should be carefully
            •  Place a 2 cm long, opened section of oesopha-  dissected, examined and sampled.
              gus in formalin.
                                                     tHInGS to notE
                                                     Thyroids
            Larynx, trachea and mainstem
            bronchi
                                                     •  Thyroids are paired glands and left and right
            •  Inspect the laryngeal aditus comprising the   thyroids should be of symmetrical and of
              epiglottis, arytenoids and vocal folds.  similar size and shape. Glands are diffusely
            •  Using scissors and forceps incise between the   soft to firm, dark red-brown, with a smooth
              arytenoids and bisect the dorsal part of the   outer contour.
              cricoid cartilage. Continue the incision down   •  Assessment of size and weight is subjective
              the dorsal ligament of the trachea and on into   as weight ranges for endocrine organs in vet-
              the mainstem bronchi.                    erinary species are not available.
            •  Locate the bronchial lymph nodes adjacent   •  Bilateral increase in size may indicate goitre.
              to the mainstem bronchi. Incise and examine   Goitre  occurs  as  a  result  of  accumulation
              the cut surface.                         of unconjugated colloid in thyroid follicles
                                                       which can occur secondary to iodine excess
            tHInGS to notE                             or deficiency.
            •  Swelling of the laryngeal mucosa, masses or   •  Iodine deficiency can occur as a result of
              foreign material which may have resulted in   absolute dietary deficiency or secondary to
              upper airway obstruction.                ingestion of goitrogenic plants or chemicals







       Vet Lab.indb   504                                                                  26/03/2019   10:26
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