Page 122 - Live-cellanalysis handbook

Live-Cell Analysis Handbook — Third Edition

Sample Data

Differential morphology of microglia
Finding the optimal in vitro model for studying microglia in the three immortalized microglia cell lines. Dramatic differential
pathogenesis of neurodegenerative disorders can be difficult. morphology was observed depending on species and source
Current options for studying microglia, include immortalized cell (Figure 1). Evaluation of imaged-based changes in morphology
lines, primary cell lines and human induced pluripotent stem cell gives insight into in vitro culture conditions, cell activation status
(hiPSC)-derived microglia. In order to evaluate morphological and reprogramming events for the development of therapeutic
characteristics of each of these models, we imaged rat primary targets for neurodegenerative disorders.
microglia, two different sources of hiPSC-derived microglia, and

Rat primary microglia Axol IPSC-derived microglia CDI IPSC-derived microglia

BV-2 C8-B4 HMC3

Figure 1. Visualization of morphological differences in microglia. Representative images from: Rat Primary microglia (Brain Bits – top left), iPSC-derived microglia
(Axol BioSciences – top middle), iPSC-derived microgia (Cellular Dynamics – top right), immortalized muring microglia cell lines BV-2 (bottom left) and C8-B4
(bottom middle), and HMC3 human immortalized microglia (bottom right).

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