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31  Canine Chronic Bronchitis  309


  VetBooks.ir    Box 31.3  Airway sampling techniques              hood in retrieving a representative cytology from more
                 Cytologic samples can be obtained from the airway via a
                 tracheal wash, blind bronchoalveolar lavage, or with
                 bronchoscopy.                                     areas of the airway.
                   Transtracheal wash (TTW) is best suited for tolerant   Endotracheal wash (ETW) is commonly performed in
                 medium‐ or large‐sized dogs.  This technique should be   smaller patients, patients that are less amenable to restrain,
                 avoided in brachycephalic breeds, and obese patients   brachycephalic breeds, and obese patients.
                 where easy palpation of the trachea is limited.
                                                                 ●   ETW is performed by briefly anesthetizing the dog with
                   TTW is performed in unsedated or lightly sedated dogs.  propofol then performing a clean intubation with a sterile
                 ●
                   After aseptic preparation of a small area over the cervical   endotracheal tube. Lubricating gel should not be used.
                 ●
                  trachea or larynx and a local anesthesia block is per-  ●   In a sterile fashion, a 5–8 Fr catheter is fed through the
                  formed, a through‐the‐needle catheter is passed through   endotracheal  tube  and  sterile  saline  is  infused  and
                  the cricothyroid ligament or between tracheal rings; the   reaspirated. Alternatively, a modified technique using
                  catheter is fed down the trachea (beveled edge down).  suction, a Lukens specimen container, a three‐way stop-
                   Two to three aliquots of 5–10 mL 0.9% sterile saline are   cock,  and  a  red  rubber  or  Argyle  feeding  tube  can  be
                 ●
                  flushed into the trachea, and promptly retrieved. Retrieval   used (Figure 31.4a,b).
                  volume is typically about 50%, and the remaining fluid is   ●   0.9% saline aliquots of 3, 5, and 10 mL are used for dogs
                  rapidly absorbed from the airway.                <5 kg, 5–15 kg, and >15 kg, respectively.
                   Collection of a diagnostic sample is facilitated by a     A sterile collection cup may be used to collect any addi-
                 ●                                               ●
                  patient’s ability to cough resulting in an increased likeli-  tional samples that may be expectorated.

                 (a)
























                 (b)

















                 Figure 31.4  Equipment set‐up for a modified technique to perform bronchoalveolar lavage or endotracheal tube lavage using
                 suction, a Lukens specimen container, a three‐way stopcock, and a red rubber or Argyle feeding tube.
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