Page 43 - Book of Abstracts

th
8 Biannual Conference on Chemistry - CHEM 08

In Vitro Inhibitory Effect of Partially Purified N-Hexane

Fraction of Tapinanthus Bangwensis on Alpha Amylase and
Glucosidase Activity

Ihegboro, G.O* , Ononamadu, C.J , Owolarafe, T.A , Afor, E , Iko, S
1
1
1
2
1
and Ndam, S
1
1 Department of Biochemistry and Forensic Science, Faculty of Science, Nigeria
police Academy, Wudil, kano, Department of chemical Sciences, School of
2
mathematics snd Sciences, Yabs college of Technology, Yaba, Lagos.
Email: goihegboro@yahoo.com

ABSTRACT
Tapinanthus bangwensis is a citrus tree dependent plant found mostly in
tropical and sub-tropical regions. It has been reported to possess
medicinal benefits against various health challenges including diabetes
mellitus. The aim of the present study was to evaluate the in vitro
inhibitory effect of partially purified n-hexane fraction of T. bangwensis
against the activity of α-amylase and α-glucosidase. The phytochemical
screening, antioxidant and inhibitory activity were determined by
spectrophotometric technique while the toxicity study was carried out by
using brineshrimp and Allium cepa models. The qualitative screening
showed the presence of flavonoid, phenol, tannin, alkaloid and cardiac
glycoside while steroid, phlobatanin, terpenoid and Saponin were not
detected. The quantitative analysis of the detected phytochemicals were
within the range of 20-31mg/100kg.The 2,2-diphenyl-1-picrylhydrazine
(DPPH) and nitric oxide assays showed significant antioxidant activity
especially at 100µg/ml, however, ascorbic acid exhibited the strongest
antioxidant activity compared to the partially purified fraction of T.
bangwensis. The inhibition concentration (IC50) for the brineshrimp is

given as 7.46µg/ml while the Allium cepa result showed decrease in
percentage mitotic index (%MI), number of dividing cells and
meristematic cells. The result also showed that as the concentration
increased, the number of chromosomal aberrations decreased. The result
showed significant inhibition of α-amylase and α-glucosidase activity
especially at 100µg/ml,, however, the inhibitory effect was higher in α-
amylase compared to α-glucosidase. From the result obtained, it can be
concluded that T. bangwensis may be effective in the treatment and
management of diabetes mellitus.
Key words: Tapinanthus bangwensis, Diabetes mellitus, Inhibitory effect, α-
amylase, α-glucosidase, Brineshrimp,spectrophotometric technique

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