Page 180 - AWSAR 2.0

156 || AWSAR Awarded Popular Science Stories - 2019
Diagrammatic representation of endometrium regeneration using PLA-VEGF: 1. A woman with thin endometrium condition and inability to conceive 2. Collection of the Endometrium Stromal Cells (hESC) from the patient’s endometrium tissues via hysterectomy 3. Production of Recombinant VEGF121 4. Encapsulation of rVEGF121 with poly-l-lactide 5. Injection of the PLA-VEGF121 into the thin endometrium (uterine lining) with the help of IUI catheter 6. After successful regeneration of the endometrium, an embryo after being prepared, being transferred in the uterine 7. A pregnant woman after successful transfer of the healthy embryo. Successful implantation lies in a fertile environment (that is, endometrium). On regenerating the endometrium there are high chances of successful implantation and pregnancy.
PLA-VEGF121 particles, which had significant differences for control vs. 200 & 500 ng of rVEGF121 and day 1, 20 &
30 of PLA-VEGF121 particles.
Tube formation is one of the experiments done worldwide to prove angiogenesis. Thus, plates were coated with MatrigelTM and seeded cells which contained rVEGF and PLA-VEGF121 as drugs. The cells were labeled with CalceinAM, a fluorescent monitoring tube formation and a cell-permeable dye; the tube formation was captured in confocal microscopy. The results were promising as they were statistically significant when compared to controls.
On proving the efficacy of
rVEGF and PLA-VEGF121 in
HUVEC these compounds were
further validated with ex-vivo angiogenesis assay (Chorio Allontoic Membrane-CAM). On evaluating the drugs in chick embryos we were
able to prove the drugs had the ability to form new blood vessels. This experiment prompted us to take up our research to the next level. So we studied the migration, proliferation, protein and the mRNA levels of human immortalized Endometrium Stromal cells (hESC) after treating the cells with rVEGF (200 and 500 ng) and PLA-VEGF121 (day 1, 20 and 30). Migration and proliferation studies in hESC proved the efficiency of the drugs treated and also they showed significant differences when compared
with the controls.
After the drugs were
treated the cells trypsinized and prepared for RNA and protein, whereas this will
be studied for the levels of the expression. Research shows that on addition of VEGF, they bind to tyrosine kinase receptors on
Endometrium thickness is one of the important factors for implantation and pregnancy. The current treatments available for endometrium regeneration is intrauterine infusion of G-CSF, which has promising results because G-CSF is a cytokine that mediates endometrium receptivity and also stimulates neutrophilic granulocyte differentiation and proliferation, which helps in endometrium regeneration.