Page 31 - IPCoSME 2021

1 INTERNATIONAL POSTGRADUATE CONFERENCE ON SCIENCE AND MARINE ENVIRONMENT 2021
st
(IPCoSME 2021)
“Environmental Sustainability Enhancement Through the Collaboration of Sciences”

RB-08

ANTIOXIDANT ENZYMES ACTIVITIES AND PROTEOMIC ANALYSIS
IN THE SCLERACTINIAN CORAL Acropora digitifera

1
NUR ATIQAH MAZNAN , SITI NUR TAHIRAH JAAFAR 1,2*

1 Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala
Nerus, Terengganu, Malaysia
2 Institute of Oceanography and Environment, Universiti Malaysia Terengganu, 21030 Kuala
Nerus, Terengganu, Malaysia

*Corresponding author email: tahirah@umt.edu.my

Abstract: Coral reefs are vulnerable to numerous stressors and temporal-related damage to
corals frequently occurred. This event could lead to degradation of coral reef and it is critically

important to understand the cellular mechanism involved in coral behind this situation. In this
study, the activity of antioxidant enzymes glutathione S-transferase (GST) and catalase (CAT)

in corals Acropora digitifera were investigated in order to understand their variations with
respect to different locations and sampling times. The corals were collected on four different

months (March, May, July and September) at three different sites of Pulau Bidong, off coast

of Terengganu, South China Sea, Malaysia. The results demonstrated variation in GST
activities with highest level in May (P<0.05) and in contrast, no significance difference in CAT

activities between different sampling months and sites (P>0.05). In addition, the coral samples
collected in Pantai Pasir Cina showed highest antioxidant activities for both enzymes

suggesting coral in this location are exposed to higher level of oxidative stress. Furthermore,
oxidative stress producing reactive oxygen species (ROS) able to modify proteins, thus, protein

analysis through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was

used for the separation and identification of proteins. Results obtained from this investigation
clearly revealed some similarities on a portion of redox proteome across different stations and

months of sampling. Some of the proteins appear randomly on certain months indicating

oxidative stress mechanism maybe common and the effects are unique for early warning
observation.

Keywords: oxidative stress, proteomics, Acropora digitifera, antioxidant enzymes, SDS-PAGE

31