Page 26 - IPCoSME 2021

1 INTERNATIONAL POSTGRADUATE CONFERENCE ON SCIENCE AND MARINE ENVIRONMENT 2021
st
(IPCoSME 2021)
“Environmental Sustainability Enhancement Through the Collaboration of Sciences”

RB-03

PHOTOSYNTHESIS AND PHOTOINHIBITION IN Sonerila picta KORTH.

1
MUHAMMAD HEEMBOO 1,2* , JAREANSAK SAE WAI AND PIMCHANOK
BUAPET 1,2

1 Division of Biological Science, Faculty of Science, PSU
2 Coastal Oceanography and Climate Change Research Center, PSU

*Corresponding author email: 6310220048@email.psu.ac.th

Abstract: The functions of the variegation and anthocyanin accumulation of leaf are still not

clear. These phenomena were found in many understory plants in the tropical rain forest,

including Senorila picta Korth. In the natural settings, we found S. picta with many forms of
leaves such as entirely green (G), variegated green (VG), entirely red (R) and variegated red

(VR). We hypothesise that variegation and anthocyanin accumulation in S. picta have a role in
photoprotection when faced with excess light. The structure of leaves was studied by freehand

cross section. Photoprotective ability was studied in control (ambient light) and high light (with
additional light sources) for eight days, then use chlorophyll fluorescence technique for

assessing photosynthesis parameters and DCF test for Reactive Oxygen Species (ROS)

analysis. We found anthocyanin accumulation in the vacuole of spongy chlorenchyma and
abaxial epidermis of red leaves (R and VR). Light area of variegated leaves (VG and VR)

displayed loosely arrangement of large size palisade mesophyll instead of normal constrict
palisade mesophyll. After high light exposure, there was no significant difference among leaf

types in maximum quantum yield (Fv/Fm), effective quantum yield (YII) and ROS
accumulation. Therefore, different leaf types do not provide a different level of

photoprotection. These findings suggest that the variegation and anthocyanin accumulation of

leaf in S. picta may not have the ability in photoprotection.

Keywords: anthocyanin accumulation, leaf variegation, photoprotection, photosynthesis, S.

picta

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