Page 24 - IBRO_RNA School_Abstract Book

Understanding developmental decision-making using

brain assembloids as models

Debojyoti Chakraborty

CSIR-Institute of Genomics and Integrative Biology, Mathura
Road, Delhi, India

Molecular pathways that determine cell fate are regulated by a concerted
integration of signals at DNA, RNA and protein levels. These include regulators with-
in the cell and extrinsic morphogenetic cues from extracellular niche. While direct
correlations between genetic and protein outputs are confounded by the abundance of
non-coding transcripts, inabilities to collectively study cellular events and extracellular

modulators in a controlled manner has made such investigations difficult in the past.
We are involved in integrating cellular and developmental sensors that can allow
such studies to be conducted in defined systems and use brain assembloids as a
developmental model to put this to test.