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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/ETERM/60
Extracts of Ipomoea carnea promote diabetic wound healing by inhibiting α-glucosidase
and stimulating cell proliferation

Muhammad Yousaf1, Rabia Mumtaz1, Muhammad Zubair1*
1Department of Bioinformatics and Biotechnology, Government College University
Faisalabad, Pakistan
Correspondence: muhammad.zubair1751@mail.com

Abstract
Diabetic wounds have always been a challenge to treat both and primary and acute care. The
present study was designed to assess the wound healing, antimicrobial and antidiabetic activity
of leaves extract of Ipomoea carnea. The various studies reported that I. carnea have potential
medicinal and pharmacological properties. The results of this research shown that ethanolic
leaves extract inhibited bacteria and α-glucosidase, also have potential wound healing
properties. The effects of using various drying temperatures for ethanolic extraction of I. carnea
leaves were reported in terms of biological activity and phytochemical constituents. Fresh
leaves of plant were washed with tap water and oven-dried at 30˚C and 60˚C and extracted in
ethanol to obtain the extracts. The wound healing activity was assessed using extracts dried
only at 30˚C. The cells treated with 5mg/ml concentration of ethanolic leaves extract of I.
carnea were given the highest cell proliferation and migration. The Klebsiella inhibition
decreases with the increase of drying temperature but in case of Acinetobacter and Bacillus,
inhibition increases with increase of drying temperature of extract. The inhibition of α-
glucosidase decreases with increase of drying temperature. The ethanolic leaves extract of I.
carnea also resulted in the highest antidiabetic activity and cell proliferation or migration in a
cell scratch assay and showed antibacterial activity too. The present study data suggests that I.
carnea leaves harbor polyphenolic compounds that have medicinal and pharmacological
properties. Future research should be conducted to explore the molecular mechanisms and
mode of action of I. carnea leaves extract.

Keywords: diabetic wound; Acinetobacter; Bacillus; polyphenolic compounds.

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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