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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/SWMR/03
Valorization of locally available waste plant leaves for production of tannase and gallic
acid by solid-state fermentation

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Shagufta Saeed and Sehrish Firyal
1 Institute of Biochemistry and Biotechnology, University of veterinary and animal sciences,
Lahore
Correspondence: shagufta.saeed@uvas.edu.pk

Abstract
To evaluate the prospective of various indigenous plant leaves for biosynthesis of value-added
products including tannase and gallic acid by Aspergillus oryzae FCBP-PTF-1202 in solid-
state fermentation. Tannic acid-rich indigenous plant leaves Psidium guajava (guava),
Syzygium cumini (black plum), Eucalyptus globulus (Eucalyptus), Ficus benghalensis
(Banyan), Citrus limon (lemon), Ficus religiosa (Sacred fig), Citrus sinensis (Orange) and
Mangifera indica (Mango) were analyzed to explore their potential as substrates for the
production of tannase and gallic acid. After assessing all these substrates, black plum leaves
produced the most promising effect resulting in a significantly (p<0.05) higher yield of tannase
(138.34 U/g) and gallic acid (0.565 mg/g). Various influential physicochemical parameters
were optimized to improve the yield of both end products. Results revealed that black plum
leaves at a substrate water ratio of 1:2, pH 5.5, and temperature 30°C yielded the best
production after 72 h of incubation period. Supplementation of glucose and nitrogen source to
basal media increased the yield of tannase (179.95 U/g) and gallic acid (0.986 mg/g).
Furthermore, the gallic acid produced was extracted by soxhlet apparatus and identified by
Fourier-transform infrared spectroscopy FTIR. The purity of gallic acid produced was 98.5%
as quantitatively analyzed by high performance liquid chromatography HPLC. In the end,
production of tannase and gallic acid from plant leaves through the optimized environment via
solid state fermentation can be exploited commercially to enhance our economy.
Keywords: gallic acid, tannase, Syzygium cumini. Aspergillus oryzae FCBP-PTF-1202.
Optimization

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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