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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/CDMP/23
Polyphenolics content and free radical scavenging potential in onion varieties cultivated
under diverse agro-climatic conditions

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Nusrat Bibi , Arshad Mehmood Abbasi *, Khalid Ahmad , Abdul Nazir , Muhammad Amin ,
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Waheed Ullah
1 Department of Environmental Sciences, COMSATS University Islamabad, Abbottabad
Campus 22060, Pakistan
Correspondence: amabbasi@cuiatd.edu.pk, arshad799@yahoo.com
Abstract
Present study was focused on impact of different environmental conditions on polyphenolics
contents and antioxidant activity in onion varieties. Total phenolics and flavonoids, and free
radical scavenging capacity were determined using different assays. While impact of growing
conditions was assessed using statistical models. Mustang (V1) variety, planted at Pattoki,
Lahore (L) and Swabi (S) had maximum TPC (6.595±0.07 and 6.311±0.09 mg GAE/100g,
respectively). Whereas, Amazon and Mustang varieties harvested from Kallarkahar had highest
TFC (5.931±0.03 and 3.030±0.07 mgQE/100g, respectively). Likewise, DPPH, H2O2 and
ABTS activities were maximum in V1 planted at KK, S and L, while Golden Orb (V6),
exhibited significant potential to scavenge the OH radical at L. Likewise, V1 from KK and S
had substantial capacity to reduce ferric and molybdate ions assessed by Phosomolybdenium
complex (PMA) antioxidant assay. Correlation and principal component analyses revealed
strong relationships of growing conditions with polyphenolics and antioxidant activities. Our
findings revealed that variations in the phytochemical contents and antioxidant potential, and
their relations with growing conditions exposed the impact of growing conditions and genetic
diversity.

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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