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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/ETERM/11
Biological control fusarium wilt disease in cotton plants by endophytic rhizobacterim:
Saccharothrix algeriensis NRRL B-24137

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Saima Muzammil* , Rizwan Asif , Muhammad Waseem , Muhammad Hussnain Siddique
1 Department of Microbiology, Government College University Faisalabad (GCUF),
Faisalabad.
1 Department of Bioinformatics and Biotechnology, Government College University Faisalabad
(GCUF), Faisalabad.
Correspondence: saimamuzammil83@gmail.com
Abstract

Cotton (Gossypium spp.) is a significant worldwide cash crop and is cultivated commercially
in over 50 nations. There are more than 60 diseases which are threating the cotton and one of
the most destructive disease caused by Fusarium oxysporum f. sp. vasinfectum is Wilt disease.
Chemical fungicides are applied to manage the wilt disease. There is a need for conservation
of the environment, rural development and the standard life style of the rural population and
the successful implementation of organic production. Alternative approaches to chemical use
are highly appreciated due to the increasing importance of integrated management strategies
relying on biocontrol. For development of environmentally friendly microbial products, current
study was designed with the aim of biocontrol study of cotton wilt disease using Saccharothrix
algeriensis NRRL B-24137. Sa. algeriensis isolated from a Saharan soil was evaluated as a
biocontrol agent in the laboratory and field conditions. In-vitro dual culture method was used
for the screening of antagonistic activities of Sa. algeriensis NRRL B-24137 against pathogenic
fungi. In-vivo experiment was also conducted for evaluation the anti-fusarium activities of Sa.
algeriensis NRRL B-24137. For in-vivo study pre-sowing cotton seeds were treated with
bacterial suspension while the control plants soaked only in CMC solution and implanted in
pots. Our results confirm that the Sa. algeriensis NRRL B-24137 have a great potential for the
control of cotton wilt under field conditions as compared to control plants and responsible for
significantly increase in the cotton yield, which a good approach to start in agricultural field.

Keywords: Cotton; Sa. Algeriensis; Biocontrol; Fusarium wilt

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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