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International Conference on

Recent Trends in Environmental Sustainability

ESCON22/ETERM/26
Utilization of agricultural wastes for the hyperproduction of Α-Amylase from
Geobacillus Sbs-4s and its application in detergent and textile industry

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Muhammad Tayyab* , Sehrish Firyal , Shagufta Saeed , Muhammad Wasim and Ali Raza
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Awan
1 Institute of Biochemistry and Biotechnology, University of Veterinary and Animal
Sciences, Outfall Road, Lahore
Correspondence: muhammad.tayyab@uvas.edu.pk
Abstract
The present study deals with the production, purification and characterization of α-amylase
using Geobacillus SBS-4S and its role in the detergent and textile industry was analyzed.
Various carbon and nitrogen sources were utilized for the supplementation of LB medium. 0
to 3% concentration of carbon sources (wheat bran, rice bran and molasses) and nitrogen
sources (peptone and yeast extract) were utilized for the supplementation of LB media for the
optimum production of α-amylase. The α-amylase was produced under optimal conditions at
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55 C under shaking conditions. 2% yeast extract and 3% wheat bran showed maximum
production of α-amylase. The α-amylase was precipitated at 40% ammonium sulphate
precipitation and this protein was purified by gel filtration column chromatography. The
purified protein was utilized for characterization. Enzyme activity was analyzed using 1%
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starch as a substrate. Enzyme showed its maximal activity at 55 C, pH 8 and in the presence
+2
of Co . The molecular weight of α-amylase was recorded by SDS-PAGE. Presence of
detergents showed an enhancing effect on the activity of alpha amylase. Moreover, releases of
reducing sugars due to hydrolysis of starch make it a suitable candidate for its use in textile
industry.

Keywords: Geobacillus, SBS-4S, Optimization, Chromatography, Carbon sources

Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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