Page 14 - Practical book PC406-MNU-2025

Mansoura National University

Faculty of Pharmacy

Clinical Pharmacy - Pharm D Program

3. Beer-Lambert Law

1. Problems on Beer-Lambert Law
2. Deviations from Beer’s law {Types & Causes}
a. Real deviation 5

b. Chemical deviation ✔ ✔ ✔ ✔
c. Instrumental deviation
3. Steps for a spectrophotometric determination
1. Factors affecting absorption spectrum
a. pH.
b. Dilution.
c. Solvent. 6 ✔ ✔ ✔ ✔

d. Temperature.
2. Applications of UV-Visible Spectrophotometry
Pharmaceutical applications of Spectrophotometry (Self learning)
Introduction to Spectrofluorimetry ✔ ✔ ✔ ✔ ✔

a. Principle of photoluminescence and Chemiluminescence
b. Fluorescence and phosphorescence 7 ✔ ✔
c. Electronic transitions
d. Deactivation or relaxation processes
1. Introduction to Spectrofluorimetry ✔ ✔ ✔ ✔ ✔

a. Differences between fluorescence and
phosphorescence
b. Fluorescence spectra and Stokes shift 8 ✔ ✔
2. Instrumentation for Fluorimetric Analysis (spectrofluorimeter)
Design of a simple portable spectrofluorimeter (Self learning)
3. Differences between Spectrophotometery and Spectrofluorimetry
1. Fluorescence Quenching
2. Factors affecting Fluorescence
3. Advantages and disadvantages of spectrofluorimetry 9 ✔ ✔ ✔ ✔ ✔

4. Applications of spectrofluorimetry
Pharmaceutical applications of Spectrofluorimetry (Self learning)