Page 15 - Practical book PC406-MNU-2025

Mansoura National University

Faculty of Pharmacy

Clinical Pharmacy - Pharm D Program

1. Introduction to Chromatography 10
2. Classification of chromatographic techniques ✔ ✔
3. Theory of column chromatography
4. Efficiency of chromatographic column separation 11 ✔ ✔ ✔ ✔

5. Origins of band broadening
1. HPLC apparatus (chromatograph)
2. Applications of HPLC in pharmaceutical analysis 12 ✔ ✔ ✔ ✔ ✔

Pharmaceutical applications of HPLC (Self learning)

1. Gas Chromatography principle and apparatus (chromatograph) 13 ✔ ✔ ✔ ✔ ✔ ✔

1. Applications of GC in pharmaceutical analysis
Pharmaceutical applications of GC (Self learning) 14 ✔ ✔ ✔

2. Advantages of HPLC over GC

Course Key Elements
Domain: 1 Domain: 2 Domain: 4
Study Week
Course contents
1.1.1.1 1.1.3.1 2.2.1.1 2.2.3.1 2.2.4.1 2.3.1.1 2.3.2.1 4.1.1.1 4.1.2.1 4.2.2.1 4.3.1.1 4.3.2.1

A) Practical part

Introduction to spectrophotometry.
1 ✔ ✔ ✔ ✔ ✔
Beer’s Lambert law.
Colorimetric determination of KMnO4 (drawing calibration
curve). 2 ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔
Colorimetric determination of K2CrO4 & regression equation by 3
calculator. ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔