Page 21 - Practical book PC406-MNU-2025

Mansoura National University

Faculty of Pharmacy

Clinical Pharmacy - Pharm D Program

1. Types of Spectrophotometer
a. Single-beam Spectrophotometer
4 b. Double-beam Spectrophotometer ✔ ✔ ✔ ✔ ✔
2. Transmittance and Absorbance
3. Beer-Lambert Law
1. Problems on Beer-Lambert Law
2. Deviations from Beer’s law {Types &
Causes}
5 a. Real deviation ✔ ✔ ✔ ✔
b. Chemical deviation
c. Instrumental deviation
3. Steps for a spectrophotometric determination
1. Factors affecting absorption spectrum
a. pH.
b. Dilution.
c. Solvent.
6 d. Temperature. ✔ ✔ ✔ ✔ ✔
2. Applications of UV-Visible
Spectrophotometry
Pharmaceutical applications of Spectrophotometry (Self
learning)
Introduction to Spectrofluorimetry
a. Principle of photoluminescence and
Chemiluminescence
7 b. Fluorescence and phosphorescence ✔ ✔ ✔

c. Electronic transitions
d. Deactivation or relaxation processes