Page 23 - Practical book PC406-MNU-2025

Mansoura National University

Faculty of Pharmacy

Clinical Pharmacy - Pharm D Program

1. Introduction to Spectrofluorimetry
a. Differences between fluorescence and
phosphorescence
b. Fluorescence spectra and Stokes shift
2. Instrumentation for Fluorimetric Analysis
8 ✔ ✔ ✔
(spectrofluorimeter)
Design of a simple portable spectrofluorimeter (Self
learning)
3. Differences between Spectrophotometery and
Spectrofluorimetry
1. Fluorescence Quenching
2. Factors affecting Fluorescence
3. Advantages and disadvantages of
9 spectrofluorimetry ✔ ✔ ✔ ✔ ✔
4. Applications of spectrofluorimetry
Pharmaceutical applications of Spectrofluorimetry (Self
learning)
1. Introduction to Chromatography
10 ✔ ✔ ✔
2. Classification of chromatographic techniques
1. Theory of column chromatography
2. Efficiency of chromatographic column
11 separation ✔ ✔ ✔ ✔ ✔
3. Origins of band broadening
1. HPLC apparatus (chromatograph)
2. Applications of HPLC in pharmaceutical
12 ✔ ✔ ✔ ✔ ✔ ✔
analysis
Pharmaceutical applications of HPLC (Self learning)
1. Gas Chromatography principle and apparatus
13 ✔ ✔ ✔
(chromatograph)