Page 40 - Practical book PC406-MNU-2025
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MANSOURA NATIONAL UNIVERSIY
         PHARM D- CLINICAL PHARMACY                        LEVEL II                       INSTRUNENTAL ANALYSIS (PC 406)



            For close imagination what happens inside the spectrophotometer while
              measuring the absorbance of substance watch these online videos




                https://www.youtube.com/watch?v=tdX8RN1X33M





               htt ps://www.youtube.com/watch?v=yTabfxvMdCM





               https://www.youtube.com/watch?v=vJCnWl            - p_d0





                                             Beer's Lambert Law











                                           Absorption of light by a sample


          Beer’s Law:
             The absorbance is directly proportional to the concentration of the absorbing

             substance.                                         A α c








          12 | P a g e                                 S e c o n d   s e m e s t e r       2 0 2 4 - 2 0 2 5
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