Page 54 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

IV- Colorimetric
determination of CuSO4

P Pr ri in nc ci ip pl le e

2+
 The colorimetric determination of CuSO 4 depends on the reaction between Cu
and ammonia that yields a blue Cu-ammine complex which can be determined

colorimetrically at max 600 nm.

2+
Cu + 4 NH3 → [Cu(NH3)4]
2+
Blue colored complex
(copper-amine complex)

P Pr ro oc ce ed du ur re e

1) Transfer 2.0, 4.0, 6.0, 8.0, 10.0 mL of standard CuSO 4 solution and 10.0 mL of

the sample solution into 100-mL measuring flasks .

2) To each flask add 2 mL of conc. ammonia solution

3) Dilute to the mark with water and mix well.

4) Measure the absorbance at max 600 nm and draw the standard calibration curve.

5) From the calibration curve determine the unknown concentration of the sample

and calculate a, 1% , ε.
 cm 1

** N.B: - Concentration of the standard CuSO4 solution = 500 mg/L
-M.Wt of CuSO4 = 160 gm.

26 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5