Page 6 - Practical book PC406-MNU-2025

Mansoura National University

Faculty of Pharmacy

Clinical Pharmacy - Pharm D Program

(4.1.2) (4.1.2.1) Retrieve and analyze information to solve problems, and work individually
or effectively in a team.

(4.2.2) (4.2.2.1) Apply contemporary technologies to demonstrate effective presentation
skills

(4.3.1) (4.3.1.1) Apply effective strategies to manage and improve self-practice of
pharmacy

(4.3.2) (4.3.2.1) Practice self-learning needed to improve professional skills

2. Course Contents:

A. Theoretical part

Week Topics Lecture
No. credit Hours

Introduction to Spectrophotometry
1 a. Principle of spectrophotometry and colorimetry 2
b. Electromagnetic radiation (EMR)
c. Electromagnetic spectrum
Introduction to Spectrophotometry
a. Interaction of light with matter
2 b. Requirement for light absorption 2
c. Absorption Spectrum
d. Spectrophotometric Measurement
Components of Spectrophotometer
a. Light Source.
b. Wavelength Selector (Monochromator).
3 c. Sample Compartment & Cuvette. 2

d. Detector.
e. Readout device.
Design of a simple portable spectrophotometer (Self learning)
1. Types of Spectrophotometer
a. Single-beam Spectrophotometer
4 b. Double-beam Spectrophotometer 2
2. Transmittance and Absorbance
3. Beer-Lambert Law
1. Problems on Beer-Lambert Law
2. Deviations from Beer’s law {Types & Causes}
5 a. Real deviation 2
b. Chemical deviation
c. Instrumental deviation
3. Steps for a spectrophotometric determination
1. Factors affecting absorption spectrum
6 a. pH. 2

b. Dilution.