Page 7 - Practical book PC406-MNU-2025

Mansoura National University

Faculty of Pharmacy

Clinical Pharmacy - Pharm D Program

c. Solvent.
d. Temperature.
2. Applications of UV-Visible Spectrophotometry
Pharmaceutical applications of Spectrophotometry (Self learning)
Introduction to Spectrofluorimetry
a. Principle of photoluminescence and Chemiluminescence
7 b. Fluorescence and phosphorescence 2

c. Electronic transitions
d. Deactivation or relaxation processes
1. Introduction to Spectrofluorimetry
a. Differences between fluorescence and phosphorescence
b. Fluorescence spectra and Stokes shift
8 2
2. Instrumentation for Fluorimetric Analysis (spectrofluorimeter)
Design of a simple portable spectrofluorimeter (Self learning)
3. Differences between Spectrophotometery and Spectrofluorimetry
1. Fluorescence Quenching
2. Factors affecting Fluorescence
9 3. Advantages and disadvantages of spectrofluorimetry 2
4. Applications of spectrofluorimetry
Pharmaceutical applications of Spectrofluorimetry (Self learning)

10 1. Introduction to Chromatography 2
2. Classification of chromatographic techniques
1. Theory of column chromatography
11 2. Efficiency of chromatographic column separation 2
3. Origins of band broadening
1. HPLC apparatus (chromatograph)
12 2. Applications of HPLC in pharmaceutical analysis 2
Pharmaceutical applications of HPLC (Self learning)

13 1. Gas Chromatography principle and apparatus (chromatograph) 2

1. Applications of GC in pharmaceutical analysis

14 Pharmaceutical applications of GC (Self learning) 2

2. Advantages of HPLC over GC

B. Practical part
Week Practical Topics Practical
No. credit hours

Introduction to spectrophotometry. 1
1
Beer’s Lambert law.
2 Colorimetric determination of KMnO4 (drawing calibration curve). 1

3 Colorimetric determination of K2CrO4 & regression equation by 1
calculator.
4 Colorimetric determination of FeCl3 & regression equation by 1