Page 63 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

Applications of spectrofluorimetry:

I- Spectrofluorimetric determination of Quinine sulfate

 The techniques of spectrofluorimetry and phosphorimetry measure the intensity of

the light emitted from a system that has absorbed radiant energy.

 Preparation of stock solution:

 Prepare a 100.0 µg/mL quinine sulfate stock solution by quantitatively transferring

exactly 10 mg quinine sulfate to 100.0 mL volumetric flask, adding 5 mL 1 M

sulfuric acid, and diluting to volume with deionized water.

 This stock solution was further diluted with 0.05 M sulfuric acid as appropriate to

obtain the working concentration range.

 Procedures:

1) Transfer aliquots of the working standard solution into a series of 10 mL
volumetric flasks.

2) Complete to the volume with 0.05 M sulfuric acid.

3) Measure the fluorescence intensity at 455 nm after excitation at 350 nm, using

0.05 M H 2SO 4 as a blank.

35 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5