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International Journal of Environment,  Agriculture and Biotechnology  (IJEAB)                              Vol-3, Issue-5,  Sept-Oct- 2018

          http://dx.doi.org/10.22161/ijeab/3.5.48                                                                                                                      ISSN: 2456-1878
            To determine fruit to sugar to yeast ratio, the experiment   Wine was harvested by siphoning carefully, filtered and
          was arranged in a 4 x 3 factorial experiment in Completely   clarified, transferred to ageing bottles and allowed to age
          Randomized Design (CRD) with level of sugar as Factor A   for 3 months.
          and  different  amounts  of  yeast  as  factor  B.  The   Wine was packed in clear wine bottles and pasteurized
          experimental  units  were  replicated  three  times  in storage   for 15 min at 75 C.
                                                                            o
          after single preparation.
          Factor  A, level of sugar (LS) per kilogram dragon  fruit   2.3  Product  Evaluation
          LS1  = 0.25  kg                                      Soluble  solids  and  alcohol  content  of  dragon  fruit  wine
          LS2  = 0.50  kg                                      samples  were  determined  right  after  fermentation.  Total
          LS3  = 0.75  kg                                      soluble solid content was determined using refractometer
                                                                               o
          LS4  = 1.0 kg                                        and  expressed  in  Brix.  Total  acidity  was  determined by
          Factor  B, Amount  of Yeast (AY)  per kilogram dragon   titration method and alcohol content of the distillate was
          fruit                                                measured using a hydrometer.
          AY1 = 1 tsp                                            Sensory  properties  of  the  dragon  fruit  wine  were
          AY2 = 2 tsp                                          determined  by  a  trained  panel.  The  wine  samples  were
          AY3 = 3 tsp                                          presented in shot glasses to the panel. Unstructured 5-point
            The following treatment combinations were used:    scale for blind testing of wines was used (Table 1).
             T       LS    Sugar, kg        AY     Yeast,
                                                    tsp           Table.1: Unstructured score sheet for blind testing of
             T1   =  LS1     0.25     x    AY1       1                             wines [3]
             T2   =  LS1     0.25     x    AY2       1          ATTRIBUTE                  SCORE
             T3   =  LS1     0.25     x    AY3       1                            5       4    3   2      1
             T4   =  LS2     0.50      x   AY1       2         Appearance    Clear,                   Cloudy,
             T5   =  LS2     0.50     x    AY2       2                       appropriate              off colors
             T6   =  LS2     0.50     x    AY3       2                       color,
             T7   =  LS3     0.75     x    AY1       3                       brilliance,
             T8   =  LS3     0.75     x    AY2       3                       no off colors
             T9   =  LS3     0.75     x    AY3       3         Aroma         Complex,                 Little or
            T10   =  LS4     1.00     x    AY1       4                       many                     no aroma,
            T11   =  LS4     1.00     x    AY2       4                       detectable               off aromas
            T12   =  LS4     1.00     x    AY3       4                       aromas,
                                                                             intense
          2.2  Processing of Dragon Fruit Wine                 Body          Perfect                  Too little
            Four  kilos  of  dragon  fruits  per  treatment  were  used.     texture and              or too
          Fruits  were  washed  in  running  water  and  drained  in         weight feel              much
          stackable plastic baskets. Each fruit was cut, peel on, into       in the mouth             texture or
          quarters  lengthwise  and  pulp  was  cut  into  1-inch  thick                              weight
          slices  crosswise before finally peeling off, separating the                                feel in the
          sliced cubes in a container. Prior to blending, the pulp was                                mouth
          diluted  with  water  at  1:2  pulp  to  water  ratio.  Pulp  was   Taste   Good            Little
          blended until homogeneous mixture  is achieved.                    balance,                 balance
            The prepared must was allowed to stand for 30 minutes            structure,               and
          to let pulp to float over juice. The juice was extracted by        several                  structure,
          scooping and separating the pulp. Sugar was added to the           flavors                  few
          extracted juice according to treatments.                           detected                 flavors
            Five  milliliters  of  10%  sodium  metabisulfite  per gallon   Finish   Flavors            Taste and
          of  must  was  added  to  sterilize  the  mixture,  allowing  24   linger after             flavors
          hours  holding  time.  Dry  yeast  was  added  according  to       swallowing,              end
          treatments.  Fermentation  of  must with cotton plug cover         smooth and               abruptly,
          was  done  for  24  hours  to  allow  yeast  incubation  and       rich after               no after
          multiplication. Fermentation bottles were then closed with         taste                    taste
          fermentation  locks.  Fermentation was allowed to proceed
          for 3-4 weeks, until gas formation ceased.



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