Page 140 - phytochemistry general program
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(1) Spraying with atomizers or aerosols.
(2) Drawing or dipping the paper in a solution of the reagent in a solvent.
(3) Some chemical detection methods need to be heated for color development.
Enzymatic and biological detection methods .3
A- Enzymatic detection methods .1
For the detection and location of enzymes or their substrates.
.2
Example: Amylases, .3
The developed chromatogram is sprayed with a starch solution, Incubated for a
suitable period,
Sprayed with iodine solution.
The location of amylases appear as white spots on a blue background.
B- Bioautographic methods .1
.1
Based on growth OR inhibition of microorganisms.
Testing growth inhibition: The chromatogram is placed on a large Petri dish with
seeded nutrients, and incubated at a suitable temperature for the specified period
of time.
The microorganisms that are sensitive to the substance analyzed grow all over
the plate, with the exception of the spots containing the test substance.
The method can also be used for growth promotion. In this case the
microorganisms grow only in areas indicated by the test substances.
Radioactive detection methods .2
A. The radioactivity can be determined by a calibrated automatic scanning device.
B. Autoradiography. The method is easier and more efficient.
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