Page 143 - phytochemistry general program

It is important at this point to make a distinction between an “organic culture” and a .1
“tissue culture”. .2
.3
An organ culture: the cultured plant material maintains its morphological identity as
a root with the same basic anatomy and physiology as the in vivo roots of the

parent plant.

Tissue culture: may be applied to any multicellular culture nurtured with a liquid
medium in which the cells are in protoplasmic continuity. The culture of an explant,
consisting of one or more tissues, results in a callus that has no structural or

functional counterpart with any tissue of the normal plant body.

Basic Plant Tissue Culture applications:

Simple plant propagation.

Synthesis of secondary metabolites.

Genetic manipulations

The basic equipment required for a tissue culture laboratory:

1- Washing and storage facilities.

2- A sterile area in a laboratory or a transfer area for aseptic manipulations

(e.g. laminar air flow cabinet), do not share area with any microbiologist or

pathologist.

3- An autoclave for media sterilization and a storage room.

4- Culture room or incubator for maintenance of cultures under controlled

conditions of temperature, light and humidity.

5- A rotary flask shaker to keep suspension cultured dispersed and aeriated.

Aseptic technique used in PTC

It is important to notify that culturing plant cells should be under sterile conditions
as the culture media in which plants grows are suitable for the growth of bacteria,
fungi and other organisms. These would release toxic metabolite, into the culture
which may inhibit growth and or interfere with the biosynthesis and biochemical

pathways of plant culture.

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