Page 7 - Quality control of pharmaceuticals (07-PA 704) practical
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III: SUBCULTURING OF BACTERIA & FUNGI
Aim: To carryout sub-culturing of bacteria and fungus
Methods of cultivation of microorganisms:
1 cell cultivation 1 colony
A) Transfer into broth = subculture or inoculation
Culture in a liquid medium is known as inoculation. It is used as refreshment of the organism, by
growing it in a fresh medium.
Using the aseptic technique, inoculate a loopful of the organism into the clear sterile nutrient
broth tube.
Incubate at 37oC for 24 hrs.
Examine visually for microbial growth “turbidity”.
Nutrient broth Incubate at 37oC Turbidity
for 24 hrs
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