Page 116 - phytochemistry II -pharmD general
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In vivo evaluation methods:
For all in vivo methods the samples that are to be tested are usually administered
to the testing animals (mice, rats, etc.) at a definite dosage regimen as described
by the respective method. After a specified period of time, the animals are usually
sacrificed and blood or tissues are used for the assay. It is one of the most rapid
test and very useful for routine analysis.
Ferric reducing ability of plasma:
The antioxidative activity is estimated by measuring the increase in absorbance
caused by the formation of ferrous ions from FRAP reagent containing TPTZ
(2,4,6-tripyridyl-s-triazine) and FeCl2Æ6H2O. The absorbance is measured
spectrophotometrically at 593 nm.
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