Page 144 - Applied Pharmacognosy
P. 144
Types of Chemical markers
• Test solution: Dissolve 200 µl of the substance to be examined in 1.0 ml of
hexane
Reference solution: To 1.0 ml of hexane , add 20 µl of linalol , 20 µl
of estragole , 20 µl of a-terpineol , 60 µl of anethole and 30 µl of
anisaldehyde.
Column:
—material: fused silica,
—size: l = 30 m, Ø = 0.25 mm,
—stationary phase: macrogol 20 000 .
Carrier gas: helium for chromatography.
Flow rate: 1.0 ml/min.
Split ratio: 1:100.
Detection: Flame ionisation.
