Page 56 - Applied Pharmacognosy

Types of Chemical markers

of Anise (B.P.)

•Spray the plate with a freshly prepared solution of phosphomolybdic
acid and heat at 120 °C for 5 min. •The spots corresponding to
anethole appear blue against a yellow background.
•In the chromatogram obtained with 2 µl of the test solution, the spot
corresponding to anethole is intermediate in size between the spots
corresponding to anethole in the chromatograms obtained with 1 µl
and 3 µl of the reference solution.
•The chromatograms obtained with the test solution show in the
lower third a blue spot (triglycerides) similar in position to the spot in
the lower third of the chromatograms obtained with the reference
solution

(triglycerides of olive oil). 2ul 3ul 1ul 2ul 3ul