Page 70 - Applied Pharmacognosy

Types of Chemical markers

of herbal mixtures

 Three 2D-TLC chromatograms are prepared under the
same analytical conditions:

1. Herbal mixture sample.
2. Authentic herbal mixture (prepared from authentic

3. Markers mixture (marker for each herbal component)

 The comparison should include:
 The number of the spots in the diagonal.
 The color of each spot sprayed with specific spray reagents. f values of the spots

corresponding to the three