Page 92 - Applied Pharmacognosy

Types of Chemical markers
drugs by HPLC (B.P)

• Sample preparation:
• sample solution of ground herbal drug is prepared by means of solid phase

extraction (SPE) with the aim of concentrating and eluting aristolochic acids if
present in the herbal drug.

• The sorbent used, the conditioning, washing and eluting solvents are all

specified in details in the pharmacopeia