Page 6 - Quality control of pharmaceuticals (07-PA 704) practical
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flasks or other containers. While in liquefied state, solid media can be added in test tubes,
which are allowed to cool and harden in a slanted position producing agar slants after
sterilization.
6. The container are now closed with cotton plugs; screw caps or cap all covers.
7. The medium is now sterilized in an auto clave usually at 121’C for 15 min and allowed to
cool to be used for the purpose.
Some heat-labile media or specific ingredients are sterilized by using filtration.
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