Page 206 - Applied Pharmacognosy

Data handling-Increase Resolution

How to assess Column Performance?

❑ An efficient column has more theoretical plates Number (N)
than an inefficient column using a standard substance.

N = 16 tR 2
W

H = 5.54 tR 2
H1/2 W1/ 2

W1/2 = 2 t • Hwhere tr is the retention t2ime of
W
the peak and w1R/2 is the width at

Areahalf-height. w is the width at the

base in units of time. 205