Page 220 - Applied Pharmacognosy

Procedures

1.Sample is injected into a hot injector port
2.Carrier gas flows through the injector to the GC Column.
3.Mark injection time (zero) on the chart (chromatogram)

Explanation:
• The sample is vapourized

• Vapour carried by mobile phase into the column.

• Interaction of solute & stationary phase

Separation based on interaction (affinity) between solute & stationary
phase

Partition & solute volatility (GLC)
Adsorption & solute volatility (GSC)

•process is repeated many times as the components migrate through the
column.

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