Page 236 - Applied Pharmacognosy
P. 236
Capillary-Open tubular column Conventional (packed) column
High resolution columns —
2-4 mm I.D and 1-4 m long
0.1 -1 mm I.D and 5-200 m long GSC or GLC
Limited lengths reduces R and N —
PLOT (GSC) —
WCOT, FSOT, SCOT (GLC) Less sensitivity —
Higher resolution (R)
Greater HETP and N Longer analysis time —
Greater sensitivity
More mechanical strength —
Shorter analysis time
Most common in separating /determining
Columns more susceptible to damage major components in a sample (prep GC)
Most common in analytical laboratory GC Greater sample capacity — 235
instruments Lower cost (can make your own)
Smaller sample capacity
Higher cost/column
