Page 57 - Applied Pharmacognosy
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Classification of Chromatographic Techniques

          According to the Mode of Retention

1. Adsorption Chromatography.

   What are the Factors affecting adsorption Chromatography ??

b. The solvent strength:               Elutropic series

• Isocratic means using the same
  solvent (single or binary solvents)
  during the development process.

• Gradient means using different       Solvent
  solvents (according to Elutropic     System
  series, in the order of increasing
  polarity) during the development     Gradient
  process.
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