Page 61 - Applied Pharmacognosy
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Classification of Chromatographic Techniques
According to the Mode of Retention
2. Partition Chromatography.
Merits Demerits
✓ It can be applied to a ✓ High surface area of the
wide variety of sample finally divided inert support
types (both polar and together with relatively low
non-polar molecules). liquid loading result in
Partition combination of partition
and adsorption.
H2O H2O
OH OH Adsorption
✓ This situation leads to tailed
OH spots.
–C C C– 60
