Page 66 - Applied Pharmacognosy
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Classification of Chromatographic Techniques
According to the Mode of Retention
4. Molecular Exclusion (Sieving) Chromatography.
✓ Stationary phase: Insoluble polymer of known
pore size (Porous).
Aqueous liquid (Gel filtration).
✓ Mobile phase
Organic liquid (Gel permeation).
✓ This technique separates
molecules by size or Mwt,
with larger solutes passing
through most quickly.
✓ There is no attractive interaction 65
between stationary phase & solute.
