Page 64 - Applied Pharmacognosy

Classification of Chromatographic Techniques

According to the Mode of Retention

3. Ion-Exchange Chromatography.

✓ Stationary phase: Insoluble polymer or resin having a charge

bearing functional group (covalently linked).
✓ Mobile phase: Liquid.
✓ Used for separation of ionized substances based on their

affinity to the ion exchanger.

Ion-Exchanger Stationary Phase SampSleolute

- Anio-n exchanger R----N+---- Cl- Phenolic sub.-
- Cati--on exchanger R----SO3------H+ Alkaloid +
-
- -
- -

Sample Stationary phase

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